Zeiss Axiovert 135M Manuel d'utilisateur

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University of Chicago Integrated Light Microscopy Core
Dr. Vytas Bindokas, Director http://digital.bsd.uchicago.edu
By: Christine Labno, Assistant Director Room: AB-129 Phone: 4-9040
Zeiss Axiovert 135 Fluorescence Microscope
Quick Guide / Operations Manual (v. 1.0 February ‘09)
The Zeiss Axiovert 135 microscope is a manual stand with the ability to collect
fluorescence and differential interference contrast (DIC) images with limited (single color) time lapse
capability. It is equipped with a CCD camera run by MicroManager, and ImageJ-based, free software
package. Examples of types of images that can be captured range from simple image capture (bright field
or fluorescence), sequential capture of up to 4 fluorescent probes, and time lapse images of a single
channel (fluorescence or DIC). The software provides user-friendly image capture with metadata
annotation and a wide range of image processing capabilities.
Filters available on slider:
position name excitation emission
1 DAPI 365-80 450/60
2 Blank -- --
3 GFP/FITC 450-490 515-565
4 Red 546 590LP
5 Blank -- --
6 DIC -- polarizer
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Résumé du contenu

Page 1

University of Chicago Integrated Light Microscopy Core Dr. Vytas Bindok

Page 2 - Shutting down the System

2 Turning on the System 1) Fire the arc lamp under the microscope table (make sure nothing else is on) 2) Turn on the brightfield bulb powe

Page 3 - System Anatomy

3 System Anatomy The Axiovert 135 is a manually operated microscope with automated digital image capture. The objectives, emission filter cubes, DIC

Page 4 - The MicroManager Software

4 dedicated excitation and band pass emission (barrier) filters that are coupled in the slider. This makes it easy to selectively excite most fluorop

Page 5

5 select from the pulldown. A histogram representation of your image will appear in the large box at the bottom of the System window, to help you deci

Page 6 - Image 5D Data Display

6 Image 5D Data Display Images captured through the “Acquire!” button are displayed with ImageJ’s Image 5D plugin. This is a different way of displa

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