University of Chicago Integrated Light Microscopy Core Dr. Vytas Bindok
2 Turning on the System 1) Fire the arc lamp under the microscope table (make sure nothing else is on) 2) Turn on the brightfield bulb powe
3 System Anatomy The Axiovert 135 is a manually operated microscope with automated digital image capture. The objectives, emission filter cubes, DIC
4 dedicated excitation and band pass emission (barrier) filters that are coupled in the slider. This makes it easy to selectively excite most fluorop
5 select from the pulldown. A histogram representation of your image will appear in the large box at the bottom of the System window, to help you deci
6 Image 5D Data Display Images captured through the “Acquire!” button are displayed with ImageJ’s Image 5D plugin. This is a different way of displa
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