Zeiss Axioplan 2 imaging and Axiophot 2 Guide de l'utilisateur télécharger pdf (Page 12)

Microscope 2

Mag.

Type

Working

distance

(mm)

Numerical

Aperture

(N.A.)

Immersion

medium

Phase ring

position

DIC

position

Plan-Neofluar

13.6

0.15

air

10x

Plan-Neofluar

5.6

0.3

air

20x

EC Plan-Neofluar

2.0

0.5

air

40x

EC Plan-Neofluar

0.21

1.3

oil

63x

Plan-Apochromat

0.19

1.4

oil

100x

Plan-Neofluar

0.2

1.3

oil

Ph3

DIC III

Objectives can be changed using the buttons on the right side of the microscope or through software

by clicking the corresponding icon in MICROSCOPE / COMMON (Figure 9).

Figure 9. Buttons for changing the objective.

5.2 Oil objectives

5.2.1 Changing between oil and air objectives

When you change between objectives, make sure you never change to an air objective while still

having oil on the sample. The air objectives are extremely difficult to clean after being smeared

with oil.

1 2 ... 7 8 9 10 11 12 13 14 15 16 17 ... 27 28

Axiovision software 1
1 General 4
2 Turning on the equipment 5
2.4 Axiocam HRc camera 7
3 Light paths 8
4 Filters 9
5 Objectives 11
5.2 Oil objectives 12
6 Placing the sample 13
7 Focusing 14
8.1 Settings 15
8.2 Bright-field imaging 16
8.3 Phase contrast imaging 16
8.4 DIC imaging 16
9 Adjusting the oculars 18
10 Acquiring images 18
10.4 Exposure time 19
10.5 White balance 20
10.6 Image resolution 21
10.7 Display properties 21
10.9 Z-stack imaging 23
11 Post processing 24
13 Exporting images 25
15 Troubleshooting 27
15.3 Microscope 28

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Zeiss Axioplan 2 imaging and Axiophot 2 Guide de l'utilisateur Page 12

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