Zeiss LSM 510 META Manuel d'utilisateur

LSM 510 MetaTraining Notes

LSM 510 Meta Training NotesMicroscope Control (cont.)Fluorescent Light ControlYou should still be in the VIS position on the Acquire tab, and have the

LSM 510 Meta Training NotesUsing the Confocalz On the Acquire tab, press LSM to access the confocal.z Press Config to open the Configuration Control d

LSM 510 Meta Training NotesSingle Trackz Select Channel Mode for normal scanning and Single Track. Follow the excitation/emission pathway in the image

LSM 510 Meta Training NotesMulti Trackz Select Channel Mode and Multi Track.z Press Add Track for each fluorescent probe.z Select excitation line, dic

LSM 510 Meta Training NotesScanning Parameters (Channels)z Under the Acquire tab, select Scan to access the Scan Control Dialogue.z Select the Channel

LSM 510 Meta Training NotesScanning Parameters (Channels cont.)z Choose Range Indicator from Palette, on the image display window, to adjust the gain

LSM 510 Meta Training NotesScanning Parameters (Mode cont.)z Switch to the Mode tab to gain access to:• Scan Speed – slower scan speed will improve i

LSM 510 Meta Training NotesNyquist Samplingz In order to preserve the spatial resolution in an image the sampling rate should be twice that of the max

LSM 510 Meta Training Notesz Improve image quality• Slow down the scan speed to allow for longer pixel dwell time.• Averaging to improve signal to noi

LSM 510 Meta Training NotesZ-Stack Settingsz Adjust settings at optimal plane of focus.z Select Mark First/Last tabz Use the fast scan setting to focu

LSM 510 Meta Training NotesTurning on the systemz Turn on X-Cite power supply. This supplies light for epifluorescence for viewing your samples throug

LSM 510 Meta Training NotesTime Seriesz Start Series – Select manual start, press the StartT icon.z Stop Series – Select either Manual or Time. • Manu

LSM 510 Meta Training NotesShutdown Procedure (Final User)z Open the Laser Control Dialog. • Argon – Lower power, select Standby, select Off. Wait unt

LSM 510 Meta Training NotesInitializing the softwarez Double click on the LSM 510 Meta icon located on the desktop.z In the LSM Switchboard, select “S

LSM 510 Meta Training NotesLSM 510 Meta ToolbarYou can easily navigate from the left to right on the toolbar. Selections from the top row will change

LSM 510 Meta Training NotesDatabase Structure and Accessing Filesz When you create a new database, the software automatically creates a new folder wit

LSM 510 Meta Training NotesThe lasersz Go to the Acquire tab and select Laser to open the Laser Control dialog that gives you access to the three lase

LSM 510 Meta Training NotesThe lasers (cont.)z HeNe 1 and HeNe 2 – The helium-neon lasers are much weaker lasers than the argon laser and do not requi

LSM 510 Meta Training NotesSelecting ObjectivesIt is easiest to define the objective(s) you will be using in the software, and then use the Microscope

LSM 510 Meta Training NotesMicroscope ControlTransmitted Light ControlOn the Acquire tab, press VIS to allow you to look through the microscope, and t