Zeiss LSM 510 META Manuel d'utilisateur

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour1Confocal microscopyZeiss LSM 510 and Zeiss LSM 510 METAVisualisa

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour101) Double click theLSM 510 icon2) Select “Scan NewImages”3) Sel

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour111) In the main menu File select New databaseCreating a database

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour121) Select AcquireTurning on the lasers2) Select Laser3) Switch

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour13For laser scanning imageacquisition:Set slider to “LSM”(pull sl

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour14Toggle between Vis and LSM button in main menu, automatic swit

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour15• Starting the Zeiss LSM 510 microscope, software and laserSele

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour161) Select Micro (Main menu: Acquire)(For Axioskop 2 FS these se

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour17Focusing the microscope in fluorescence modeFluorescence is obs

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour18Focusing the microscope in transmitted modeUse no reflector cub

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour19• Starting the Zeiss LSM 510 microscope, software and laserSele

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour2Confocal PrincipleSignals from aboveand below the planeof focus

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour20Choosing the configurationMULTI TRACKUse for double ortriple la

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour211) Select Config in theAcquire menuSINGLE TRACK - lasers scan s

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour225) Chose a configuration in theTrack Configuration menu.Select

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour23Multi Track Configuration2) Select Config1) Select Multi Track

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour24Cy5-Cy3-FITC Multi TrackThree laser lines and channels activate

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour251) Select ScanSetting the parameters for scanning2) Select Mode

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour26Setting the parameters for scanningNote: When using aAxioskop 2

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour27Adjust the scan speed - ahigher speed with averagingresults in

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour28Select the dynamic range - 8bit will give 256 gray levels,12 Bi

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour29Channel Settings - Adjusting the PinholeSet pinhole size to 1

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour3Upright Zeiss LSM 510 confocal microscopeLSM 510 scan headAxiop

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour302) Select Fast XY for continuousfast scanning - useful forfindi

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour31Minimal Pixel Size determined by Nyquist SamplingAdjusting the

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour32Optical ZoomingThe level of zoom can bechanged either by using

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour33Selecting gain and offset – Choosing a lookup table1) Select Pa

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour34Detector gain determines thesensitivity of the detector bysetti

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour35Laser transmission should not be set higher than the saturation

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour36Adjusting the Laser Intensity1) Set Pinhole to 1 Airy unit2) Se

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour37Adjusting Gain and Offset1) Increase the Amplifier Offsetuntil

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour38Adjusting the Laser, Gain and Offset using a Multi Track Config

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour39Setting up Gain and Offset - Multi Track1) Select Split XY in

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour4Inverted Zeiss LSM 510 confocal microscopeLSM 510 scan headin ba

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour40Line Averaging2) Select Number for averaging. Themore the bette

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour41Frame Averaging1) Select FrameFrame averaging helps reducephoto

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour42Collecting an Averaged Image1) Under Scan Average selectthe Num

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour43• Starting the Zeiss LSM 510 microscope, software and laserSele

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour44Scanning a Z-Series using Mark First/Last1) Select Z Stack2) St

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour45Using Auto Z Brightness CorrectionAuto Z provides an automatic

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour46Confocal Z SectioningNumber of Sections for correct samplingOpt

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour47Z Stack – Number of Slices and Increment1) Select Z slice - the

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour48Z - Series using Z Sectioning1) Select Z Stack2) Select Z Secti

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour491) Decide whether to Keep Interval(number of slices will change

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour5Upright Zeiss LSM 510 META confocal microscopeAxioplan 2 imaging

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour50Viewing a Z - SeriesIn the image window1) Select xy2) Select S

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour51Viewing a Z - Series using Gallery1) Select Gallery2) Select Da

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour52Viewing a Z- Series using Orthogonal Sections1) Select Ortho2)

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour53Selecting and Saving a Region of Interest (ROI)1) SelectOverlay

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour54Using a ROI for faster image acquisition and data saving1) Sele

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour55Multiple Regions of Interest1) Un-select Fit Frame Size to boun

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour56Time Series• Set up scanning parametersfor image acquisition as

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour57Viewing a Time Series of a Z StackZ Sectionsfor any timeTime po

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour58Time Series – Physiology Experiments1) If required, usemultiple

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour59Imaging a large area using Tile ScanThis function is onlyavaila

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour6Inverted Zeiss LSM 510 META confocal microscopeAxiovert 200MLSM

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour60Tiled ImageAny position can thenbe marked and asingle image acq

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour61• Starting the Zeiss LSM 510 microscope, software and laserSele

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour62Saving Data - Using Database1) Select Save or Save as on image

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour63Saving Data – Using Export1) Select File from LSM menu2) Select

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour64Shut Down Procedure 1. Go to: Acquire in the LSM menu - Laser

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour65Please note:This guided tour is intended merelyas a quick intro

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour66This guided tour is based onwork done byPeter JordanICRFLondonU

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour7• Starting the Zeiss LSM 510 microscope, software and laserSelec

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour8• Starting the Zeiss LSM 510 microscope, software and laserSelec

Carl Zeiss - Training Application and Support CenterZeiss LSM 510 META - Guided Tour91) First switch on the mercury lamp2) Turn on the remote control