Zeiss Axiovert 200 Guide de l'utilisateur télécharger pdf (Page 10)

As a last step, adjust the iris diaphragm using the slider above the DIC condenser (Fig. 5).

First, fully open it and then, if needed, close it as much as necessary for enhanced contrast.

When you are done with DIC imaging, remember to turn the polarizer away from the light

path and push the analyzer back in.

3. Imaging

3.1 Zeiss AxioCam HRc 14-bit color CCD camera

Turn the camera on by plugging the power cable in (Fig. 12). The green light indicates that

the camera is on. Alternating green and red light means that imaging is in progress.

The camera is coupled with the microscope with a 0.63x adapter.

Figure 10

Back side of the Zeiss AxioCam HRc

camera

3.2 Zeiss AxioVision software (version 3.1)

Log on to the computer with your personal HYAD-username. Choose ‘Log on to ATKK’. Start

the software by double-clicking the AxioVision-icon on the desktop. The camera should be

turned on before starting the software because it is only recognized during start-up. You can

access the online manual for AxioVision by pressing the F1 key.

3.2.1 Live view

Turn the left VIS/FP dial to FP position, so that the light will go to the camera (see "Settings

for light path", p. 3). Enable real-time video by clicking the LIVE-button in software (Fig. 11).

Figure 11 LIVE-mode button in AxioVision software

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Zeiss Axiovert 200 Guide de l'utilisateur Page 10