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The recommended minimum camera resolutions for each objective are:
Objective NA Recommended min.
resolution
10x 0.3 3900 x 3090
20x 0.4 2600 x 2060
40x 0.75 2600 x 2060
40x 0.6 1300 x 1030
63x 0.75 1300 x 1030
If you are using unnecessarily high camera resolution, the image file size will be high but no
added image resolution will be achieved. This is because the resolving power of the
objective will become a limiting factor. On the other hand, if too low camera resolution is
selected, the objective's full resolving capacity is not utilized. In practice, using higher
resolution than the recommended one will often improve the resolution as the calculations
are only approximations and the settings are usually suboptimal.
3.2.2.4 Selecting a particular area for imaging
If you want to image a particular area in your live image, go to CAMERA/FRAME and tick the
box USE FRAME. Click REFRESH OVERVIEW. Click SELECT FRAME and select some size for the
area (you can change it later). The selected area is outlined with a white frame in the live
image in the small box on the left. You can move the selected area by dragging it, and you
can change its size. Next time you take a snapshot, only the selected area will be imaged.
3.2.2.5 Acquiring a single image
A single image can be acquired by clicking the snap-button (Fig. 14). The new image will
open in a new window.
Figure 14 Single image acquisition
3.3 Display properties
AxioCam-camera can detect up to 16384 separate intensity levels for each color channel.
However, computer monitors can display only 256 levels. The way how 16384 intensity
levels are mapped to 256 can be adjusted. Click the image with right mouse button and
choose PROPERTIES (or press ALT+ENTER). Then choose DISPLAY (on the left). The black
curve describes how intensity levels are shown on display. The curve can be modified either
directly by dragging its handles with mouse, or adjusting the scrollbars for brightness,
contrast, and gamma (shape of the curve that connects the input and output intensity
values). There are also options for predefined adjustments:
(118 pages)
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