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When you click with the right mouse button and select VIEW ANNOTATIONS, you will
remove the scale bar. If you select VIEW ANNOTATIONS again, the scale bar will appear
again. The annotations including the scale bar are stored in the meta data on a “.zvi” image.
On other image file formats if you want the scale bar in the saved image, you must select
BURN-IN ANNOTATIONS option before you save your image (see the following chapter).
4. Saving and transferring images
4.1 File formats
It is recommended that you will save your images either in ZVI format. If you use TIFF, only
the image is saved, not the image acquisition parameters. However, almost any other
imaging software (e.g. Photoshop) will be able to open a TIFF file. Also, your image data will
remain unaltered.
The ZVI format is specific for Zeiss AxioVision software. In addition to the image itself, also
the imaging parameters will be stored. However, only some other imaging software will be
able to open ZVI files. SlideBook can open ZVI files, as well as the free AxioVision LE that can
be downloaded from the Zeiss web site. For more information, see MIU web site
(www.miu.helsinki.fi
).
The AxioCam camera uses 14 bits per each color channel (red, blue, green) for digitization,
and the images are saved as 3 x 16 bit in ZVI format. If you want to save your images in TIFF
format, they will be converted to 24 bpp (bits per pixel) = 3 x 8 bit so that e.g. Photoshop will
be able to handle your images.
4.2 Back-up copies
Please make sure that you always have back-up copies of your imaging data! One useful
option is to save your images in TIFF format for further image processing, and archive the
same original images in ZVI format.
4.3 Saving images
Images can be saved with FILE/SAVE AS. If you want to save several images, you can use
FILE/EXPORT and then select SEQUENCE (displayed images). In FILE/SAVE AS, you can select
in OPTIONS whether you want to apply display adjustments and burn-in annotations to your
saved images.
4.4 Saving images in Mcbserver1
This is the recommended way for all users that are affiliated with either the MCB or GSB
research programme: create a new folder with your name in the Images-folder in
Mcbserver1 and save your images directly there. You can access the Images-folder from the
microscope computers by selecting Images on Mcbserver (I), when you are saving your
images and you have to select where to save them.
(118 pages)
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