Zeiss LSM 510 Manuel d'utilisateur

Quick Start Zeiss LSM 510

10 7. Optimizing the settings Due to the sequential nature of ‘Multi-track’ acquisition optimizing the settings in this mode is difficult. It is ea

11 Your image window will now look like this.

12 7.2. Scan control: Channels window Click on the Channels button of the Scan control window. The active channel will appear here. The se

13 You need to set the detector range to match the dimmest and brightest signal from the specimen. Setting the detector incorrectly results

14 A few red speckles; a few blue speckles After you have set both channels, stop scanning by pressing the Stop button in the Scan Control wi

15 7.3. Acquiring your final image Once you are satisfied that each channels detector is set optimally to the range of the image, you can create y

16 Click on the Single button in the scan control window to collect your final image (bottom). Click on the image window Info button.

17 8. Saving your final image When you are satisfied with your image you need to save it to your database. Images are saved to Databases. A databas

18 9. Acquiring a Z-series Having set the system to acquire a satisfactory image, you can acquire a z-series. It may be worthwhile changing the fra

19 In the Scan control window, click the Z-slice button to bring up the Optical Slice dialog Click the Optimal Interval button. Check the op

2 Table of Contents 1. START HARDWARE...

20 10. Advanced Options 10.1. Single-Track – simultaneous acquisition Multi-track can solve the problem of cross-talk. Typically this occurs wit

21 10.2. Z-attenuation compensation As images are collected deeper in to the sample there can be significant loss of signal. This can be caused bu

22 DIC BF DF Ph 10.3. Transmitted light image Whilst the laser is scanning the field of view a certain amount of the excitation laser light pass

23 10.4. FRAP 1. Click on Edit Bleach toolbar button to open up Bleach Control dialog. 2. Set Bleach parameters in Bleach Control dialog Che

24 Experimental progress shown here. Progress bar will pause during the bleach process. Bleached area 7. Save experiment 8. Creat

25 11. Shutting down the system 11.1. Turn off lasers Click the Acquire toolbar button, the Laser button in the sub-toolbar. In the Laser control

26 12. Opening your images offline 12.1. Zeiss image browser http://www.zeiss.de/lsm Follow link in lower right hand corner: “Free LSM Image Bro

3 1. Start Hardware • Turn on the mercury short arc lamp light switch. Note: Whenever the mercury lamp is turned on, it should be left on for at

4 2. Start software • Log on to Win2000 (you will be issued with a username and password during training). Change your password now if you have n

5 3. Start Lasers Click the Acquire button on the toolbar. The lower toolbar will now change to the Acquire sub-toolbar and show the acquisition c

6 4. Find the specimen (Axioplan 2) The light path select, focus knob and stage are manual controls. The rest of the microscope is controlled by

7 4. Find the specimen (Axiovert 200M) • Change the microscope-light path to direct the emitted fluorescence is sent to the eyepieces by clickin

8 5. Confocal filter set configuration Click the Config button (in the Acquire sub-toolbar). To activate multi-tracking simply chose the

9 6. Acquire preliminary confocal image Click the Scan button in the Acquire sub-toolbar to open then Scan control window. Click the Find