Zeiss LSM 510 Manuel d'utilisateur télécharger pdf (Page 25)

11. Shutting down the system

11.1. Turn off lasers

Click the Acquire toolbar button, the Laser button in the sub-

toolbar.

In the Laser control window turn each laser Off or to Standby

if somebody is using the system within the next hour.

If you have used the UV laser, switch off the black switch in the

white frame located on the front of the UV laser power supply.

Do not touch any of the cooling unit settings.

11.2. Remove Specimen and clean

microscope

Wipe off water from objective and specimen.

Move to a low power objective (5× or 10×) objective. Raise the

stage using the buttons on the left had side of the microscope base.

If you switch off the system while the stage is lowered the ‘top’ of the focus range will be

reset to that position when the microscope is next turned on. This will mean the ‘top’ will

need resetting and could result in damage to the $12,000 63× objective.

Turn off the epifluorescence lamp.

Cover the microscope avoiding the hot lamp housing.

11.3. Exit the software

Exit the Zeiss LSM .

A message will come up reminding you not to power down the system until the laser is

cool. Click OK.

If you have left the lasers on for the next user, you will also be asked whether you want

the lasers switched off. Click No.

Read then close the WCIF Exit screen.

Burn your data to CD or copy across network (once installed).

Once you have finished with the computer, LOGOUT. If you do not logout, the system

will continue to charge time to your account.

11.4. Power down the system

If nobody has booked for the next hour, please shutdown the system If the next

person is the last booking of the day, please call them and confirm that they will

be using it. This requires that the remote control be switched off and the

compressed air shut down.

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Zeiss LSM 510 Manuel d'utilisateur Page 25