Zeiss LSM 510 META Spécifications Page 179

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OPERATION
LSM 510 Analysis of Images and Stacks
B 40-051 e 07/98 4-121
(21) Display - Coloc.
The Display-Coloc. function presents a comparison between two images by computing a scatter diagram
(colocalization).
How a scatter diagram is generated:
All pixels having the same positions in both images are considered a pair. Of every pair of pixels (P1, P2)
from the two source images, the brightness level of pixel P1 is interpreted as X coordinate, and that of
pixel P2 as Y coordinate of the scatter diagram. The value of the pixel thus addressed is increased by one
every time, up to the maximum number of pixels used. This way, each pixel of the scatter diagram is a
value that shows how often a particular pair of pixels has occurred.
Differences between the images cause irregular spots in the scatter diagram.
Fig. 4-129
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